The Biodiversity Lab in Poppy Playtime Chapter 5 is one of those areas that looks confusing at first, but makes a lot of sense once you know what to do. There are collectibles to grab, vines to deal with, and a battery puzzle that trips up a lot of players. This guide walks you through all of it, step by step.

Exploring the Lab and Finding Collectibles

Before you rush into the main objectives, take a moment to look around. The Biodiversity Lab has two notes hidden here, and you do not want to miss them.

The first one is easy. Head down the stairs on the left and pick up the “Lab Report: Medium Growth & Plantlife” note sitting right there.

The second note requires a bit more effort. Head back up the stairs and look for a vent behind the glass cover on your right. Break it using the Pressure Hand and go inside.

Crawl through the vent, turn left, then grapple onto the handlebar to cross the gap. Turn left again.

Inside the room, look for a whiteboard with the “Reminder” note pinned to it. Pick it up, then exit by opening the red door using the scanner next to it.

How to Use the Tank and Open the Door

Back downstairs, use your Pressure Hand to blow up a sack and remove a branch. Then push the box out of the way. Behind it, you will find a Tank.

Here is how to use it:

  1. Press the Green Pad to start filling the Tank with pressure
  2. Watch the gauge, each Tank has two lines marking a target zone
  3. Once the pressure sits between those two lines, pull the Lever
  4. The lever locks in place and activates the door

Dealing With the Vines and the Red Door

In the next section, start by turning off the two valves. This is important before you touch anything else.

Now you need to clear the vines blocking the double red door. Here is how it works:

  • Use your Pressure Hand to break the Saps in one area of the vines
  • Once enough Saps are broken, snap off the branch
  • Keep doing this across all vine sections until every Sap and branch is gone
  • Finally, break the large Sap in the middle, the red door opens

Once you are through, head into the middle room. Check the desk on the right and grab the “Dr. Arkin’s Personal Letter 1” note. Do not skip this one.

Solving the Battery Door Puzzle

Next, enter the yellow door on the right. This is where the battery puzzle takes place. Grab the first Battery Pack from the left corner of the floor.

With the Battery Pack in hand, climb the boxes and enter the vent. Jump down and grab the second Battery Pack with your right hand. Place both batteries into the slots on the right-hand wall.

Now for the actual puzzle:

  • Connect one hand to the electrical charge
  • Wrap that hand around a pillar twice
  • Without disconnecting, do the same with the other pillar
  • Once both pillars are wrapped, use your free hand to touch the end of the circuit on the opposite side of the wall

This will open the red door. Open it to meet the friendly Giblet again and progress further into the story.